diff --git a/packages/vite/src/node/__tests__/fixtures/scan-jsx-runtime/entry-glob-custom-oxc.tsx b/packages/vite/src/node/__tests__/fixtures/scan-jsx-runtime/entry-glob-custom-oxc.tsx
new file mode 100644
index 00000000000000..178cb0b9e87bff
--- /dev/null
+++ b/packages/vite/src/node/__tests__/fixtures/scan-jsx-runtime/entry-glob-custom-oxc.tsx
@@ -0,0 +1,3 @@
+export const pages = import.meta.glob('./pages/*.tsx')
+
+export default <div />
diff --git a/packages/vite/src/node/__tests__/fixtures/scan-jsx-runtime/pages/index.tsx b/packages/vite/src/node/__tests__/fixtures/scan-jsx-runtime/pages/index.tsx
new file mode 100644
index 00000000000000..1bdcfef72ff16e
--- /dev/null
+++ b/packages/vite/src/node/__tests__/fixtures/scan-jsx-runtime/pages/index.tsx
@@ -0,0 +1 @@
+export default <main />
diff --git a/packages/vite/src/node/__tests__/scan.spec.ts b/packages/vite/src/node/__tests__/scan.spec.ts
index b79930393913d3..6658425dd5dd96 100644
--- a/packages/vite/src/node/__tests__/scan.spec.ts
+++ b/packages/vite/src/node/__tests__/scan.spec.ts
@@ -175,6 +175,49 @@ test('scan jsx-runtime', async (ctx) => {
   expect(mod1).toBe(mod2)
 })
 
+test('scan import.meta.glob respects rolldown transform jsx options', async (ctx) => {
+  const server = await createServer({
+    configFile: false,
+    logLevel: 'error',
+    root: path.join(import.meta.dirname, 'fixtures', 'scan-jsx-runtime'),
+    oxc: {
+      jsx: {
+        runtime: 'automatic',
+        importSource: 'react',
+      },
+    },
+    optimizeDeps: {
+      force: true,
+      noDiscovery: false,
+      entries: ['./entry-glob-custom-oxc.tsx'],
+      rolldownOptions: {
+        transform: {
+          jsx: {
+            runtime: 'automatic',
+            importSource: 'vue',
+          },
+        },
+      },
+    },
+  })
+  ctx.onTestFinished(() => server.close())
+
+  const { cancel, result } = scanImports(
+    devToScanEnvironment(server.environments.client),
+  )
+  ctx.onTestFinished(cancel)
+
+  const scanResult = await result
+
+  expect(scanResult).toMatchObject({
+    deps: {
+      'vue/jsx-dev-runtime': expect.any(String),
+    },
+    missing: {},
+  })
+  expect(scanResult.deps).not.toHaveProperty('react/jsx-runtime')
+})
+
 test('scan import.meta.glob package imports patterns', async (ctx) => {
   const server = await createServer({
     configFile: false,
diff --git a/packages/vite/src/node/optimizer/scan.ts b/packages/vite/src/node/optimizer/scan.ts
index be05905a77c54c..06af7aaaecbf48 100644
--- a/packages/vite/src/node/optimizer/scan.ts
+++ b/packages/vite/src/node/optimizer/scan.ts
@@ -3,6 +3,7 @@ import fsp from 'node:fs/promises'
 import path from 'node:path'
 import { performance } from 'node:perf_hooks'
 import { scan } from 'rolldown/experimental'
+import type { TransformOptions as OxcTransformOptions } from 'rolldown/utils'
 import { transformSync } from 'rolldown/utils'
 import type { PartialResolvedId, Plugin } from 'rolldown'
 import colors from 'picocolors'
@@ -252,9 +253,6 @@ async function prepareRolldownScanner(
   const { plugins: pluginsFromConfig = [], ...rolldownOptions } =
     environment.config.optimizeDeps.rolldownOptions ?? {}
 
-  const plugins = await asyncFlatten(arraify(pluginsFromConfig))
-  plugins.push(...rolldownScanPlugin(environment, deps, missing, entries))
-
   const transformOptions = deepClone(rolldownOptions.transform) ?? {}
   if (transformOptions.jsx === undefined) {
     transformOptions.jsx = {}
@@ -267,6 +265,19 @@ async function prepareRolldownScanner(
   if (typeof transformOptions.jsx === 'object') {
     transformOptions.jsx.development ??= !environment.config.isProduction
   }
+  const transformSyncJsxOptions: OxcTransformOptions['jsx'] =
+    transformOptions.jsx === false ? undefined : transformOptions.jsx
+
+  const plugins = await asyncFlatten(arraify(pluginsFromConfig))
+  plugins.push(
+    ...rolldownScanPlugin(
+      environment,
+      deps,
+      missing,
+      entries,
+      transformSyncJsxOptions,
+    ),
+  )
 
   async function build() {
     await scan({
@@ -343,6 +354,7 @@ function rolldownScanPlugin(
   depImports: Record<string, string>,
   missing: Record<string, string>,
   entries: string[],
+  jsxOptions: OxcTransformOptions['jsx'],
 ): Plugin[] {
   const seen = new Map<string, string | undefined>()
   async function resolveId(
@@ -397,6 +409,7 @@ function rolldownScanPlugin(
     // transpile because `transformGlobImport` only expects js
     if (loader !== 'js') {
       const result = transformSync(id, contents, {
+        ...(jsxOptions !== undefined ? { jsx: jsxOptions } : {}),
         lang: loader,
         tsconfig: false,
       })